RESEARCH IN ENVIRONMENT AND LIFE SCIENCES

Volume-11, Number: 10 (October), 11 (November), 12 (December) – 2018

 

(A Multidisciplinary, Peer reviewed / Referred Research Journal Concerned with Environment and Life Sciences)

(p-ISSN: 0974-4908)

 

 

 

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54

Res. Environ. Life Sci., 11 (10) 265-267 (2018)

Efficacy of some medicinal plants on growth and development of pulse beetle Callosobruchus maculatus Fab.

Archana Kumari* and Sangeeta Avasthi

Department of Zoology, A.N.D.N.N.M. Mahavidyalaya, Kanpur-208012, Uttar Pradesh, India

*Corresponding author e-mail: archanamn15@gmail.com

Paper received: 22.08.18; Revised received: 26.09.18; Paper Accepted: 28.09.18; Category: Original paper

 

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Abstract: Callosobruchus maculatus Fab. is one of the major pest of pulses. Great damage is noted during storage. Post harvest losses in gram grains ranged from 30-40% within the six months due to pest attack. Many medicinal plants and spices have been traditionally used for pest control. Present study was carried on, to investigate the efficacy of six medicinal plants’ parts viz., the leaves of Boerhavia diffusa L., (punarnava), Enicostemma hyssopifolium (chhota chirayata), Merriemia turpethum L. (nishothara), Cinnamomum tamala (tejpat), flowers of Mesua ferrea (nagkesar) and root of Clerodendrum serratum L. To find out the effect of extracts of plants’ parts on growth and development of beetle, observations for fecundity, hatching percentage, pupation percentage, percentage of adult emergence and longevity of male and female adults were recorded. The minimum fecundity (13.22 eggs per female), hatching percentage (20.21%), pupation percentage (13.21%), percentage of adult emergence (4.13%) and longevity of male (2.73 days) and female (3.07 days) were recorded in the grains treated with the extract of leave of Enicostemma hyssopifolium. The grains treated with the extract of leave of Cinnamomum tamala showed least efficacy against the pulse beetle with maximum fecundity (56.13 eggs per female), hatching percentage (57.80%), pupation percentage (58.35%), Percentage of adult emergence (51.21%) and longevity of male (5.02 days) and female (6.23 days). The extracts of all taken medicinal plants used as grain protectants protect the grains from insect significantly in comparison to untreated control.

Keywords: Callosobruchus maculatus Fab., Fecundity, Hatching percentage, Adult emergence, Longevity of adults

55

Res. Environ. Life Sci., 11 (10) 268-273 (2018)

Neutralization and phytoremediation of alkaline bauxite residue (Red mud) - A review

Sonali Acharya, Rojali Maharana and Nabin Kumar Dhal

Environment and Sustainability Department, CSIR-Institute of Minerals and Materials Technology Bhubaneswar-751013, India

*Corresponding author e-mail: rojalimaharana111@gmail.com

Paper received: 11.08.18; Revised received: 21.09.18; Paper Accepted: 23.09.18; Category: Review paper

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Abstract: Bauxite residue (red mud) is a harmful waste liberated from alumina refining industries and possesses a serious environmental concern. If it is not managed properly, extreme alkalinity and its potential impact poses a significant risk to the surrounding. The proper management of bauxite residues is becoming a worldwide problem. So, its awareness must be created to protect our environment. As the amount of RM increases, it possesses a serious environmental problem. This paper describes the production of bauxite, its world and Orissa scenario, generation of red mud and its characterization. It reviews comprehensively the disposal and neutralization methods of red mud and gives the detailed idea of the work carried out til now. After neutralization, it can subsequently be treated by phytoremediation where plant growth and its accumulation capability has to take into consideration. Phytoremediation is the most promising idea for the management of bauxite residues by choosing proper vegetation.

Keywords : Bauxite residue (redmud), highly alkaline, neutralization, phytoremediation

56

Res. Environ. Life Sci., 11 (10) 274-276 (2018)

Companion cropping of summer groundnut in spring planted sugarcane for increasing two fold income of farmers

R.A. Singh1*, M.K. Singh1, Subhash Chandra1 and I.P. Singh2

1C.S. Azad University of Agriculture and Technology, Kanpur (U.P.), India

2K.V.K., Auraiya, (U.P.), India

*Corresponding author e-mail: rasinghcsau@gmail.com

Paper received: 17.08.18; Revised received: 19.09.18; Paper Accepted: 24.09.18; Category: Original paper

 

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Abstract: The study was conducted during 2006 and 2007 in the catchments area of river Ganga at Unnao district of central Plain Zone IV of Uttar Pradesh. The experimental soil was sandy clay loam, having poor fertility status. The main objective was to popularize the summer groundnut cultivation as intercrop with spring season planted sugarcane for increasing income of farmers upto two fold. The highest pod yield of groundnut was recorded with cultivar Dh 86 and ICGV 93468 by 27.40 q/ha and 27.35 q/ha, respectively, in inter cropping system. the order of varietal performance was Dh 86 and ICGV 93468 followed by ICGS 1, ICGS 44, ICGV 86590 and G 201 in companion cropping of sugarcane and summer groundnut without any adverse effect of sugarcane on summer groundnut and vice-versa. Spring sugarcane intercropped with different cultivars of summer groundnut produced higher cane stalk over spring sugarcane alone. The spring sugarcane intercropped with Dh 86 and ICGV 93468 gave higher stalk yield by 782.00 q and 783.00 q/ha, respectively, in comparison to other cropping system and spring cane alone (761.00 q/ha). the companion cropping system with cvs. Dh 86 and ICGV 93468 increased the income by 1.95 fold over spring sugarcane alone.

Keywords: Companion cropping, Summer groundnut, Synergistic cropping, two fold income

57

Res. Environ. Life Sci., 11 (10) 277-280 (2018)

Effect of nickel and its interaction with boron on pigments and enzymes activity in Pisum sativum seedlings

A. Verma*

Department of Botany, University of Lucknow, Lucknow-226 007, India

*Corresponding author e-mail: adi_kobe@yahoo.co.in

Paper received: 22.07.18; Revised received: 06.09.18; Paper Accepted: 08.09.18; Category: Original paper

 

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Abstract: The pea seedlings were raised in petridish for 15 days in different concentrations of nickel (100 µM, 200 µM, 400 µM and 600 µM). Combination of boron (50 and 100 µM) with nickel (200, 400 and 600 µM), was also given for the recovery of damage occurred in pea seedlings. In nickel treatments (100, 200, 400 and 600 µM), chlorophyll (a, b, and total) and pheophytin (a, b, and total) contents were found to be decreased in comparison to control. In control total chlorophyll and total pheophytin were 1.527 mg/g and 1.348 mg/g but they were 1.493 and 1.358 mg/g in 100 µM nickel, 0.990 and 1.297 mg/g in 200 µM nickel, 0.656 and 1.126 mg/g in 400 µM nickel and 0.375 and 1.071 mg/g in 600 µM nickel. On combined application of boron (50 and 100 µM) these pigments were increased. Carotenoids content was increased (0.216, 0.236, 0.263 and 0.286 mg/g) with increasing nickel concentrations from control (0.207). In the recovery treatments of boron, carotenoids contents were found to be decreased at certain levels. The amylase activity (a, b and total amylase) was inhibited in all the nickel concentrations (100, 200, 400 and 600 µM) except a very slight increase in â amylase in 100 µM and 200 µM nickel treatment. In combination with boron (50 and 100 µM) amylase activity increased to certain level and maximum 8.50 amylase activity was analyzed in recovery treatment of 50 µM boron in 200 µM nickel. Marked increase in catalase and peroxidase activity was noticed in seedlings on exposure of nickel over control. Similar trend was observed in the activities of acid phosphatase and polyphenol oxidase. In recovery treatment of iron 50 µM in 200 µM nickel, acid phosphatase and polyphenol oxidase showed better recovery than in other recovery treatments.

Keywords: Pea, Nickel, Boron, Iron, Cholorophyll, Pheophytin, Carotenoids, Amylase, Catalase, Peroxidase, Acid phosphatase, Polyphenol oxidase

58

Res. Environ. Life Sci., 11 (10) 281-284 (2018)

Study of biochemical activity and bioaccumulation in Spinacia oleracea L., as treated by tannery effluent with effect on soil properties

Kamlesh Nath1*, B.P. Singh2 and Y.K. Sharma2

1E.C.S., A.R.P. Ltd.Lucknow, India; 2Department of Botany, Lucknow, University, Lucknow-226007, India

*Corresponding author e-mail: nathkamlesh@rediffmail.com

Paper received: 28.07.18; Revised received: 14.09.18; Paper Accepted: 11.09.18; Category: Original paper

 

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Abstract: Effect of tannery effluent on total proteins, total sugar, catalase and peroxidase was studied in spinach. The protein increased at lower levels of tannery effluent and then significantly decreased at >50% tannery effluent onwards. Sugar decreased significantly as tannery effluent concentration increased. Peroxidase ,Catalase was decreased at 10% to 25% tannery effluent and showed significant increase at 50 % tannery effluent onwards. In recovery treatments protein and catalase were higher with 25 ppm of zinc sulphate, potassium sulphate and iron sulphate when combinedly used with 50% tannery effluent. Sugar was observed higher with 25 ppm of zinc sulphate, iron sulphate and 10 ppm of potassium sulphate whereas peroxidase was higher with 10 ppm of zinc sulphate and iron sulphate and 25 ppm of potassium sulphate when combinedly used with 50% concentration of tannery effluent. All parameters of soil showed increase with increased concentration of tannery effluent. In recovery treatments pH was higher with 10ppm of zinc sulphate, potassium sulphate and iron sulphate whereas EC was higher with 25 ppm of zinc sulphate and potassium sulphate and 10 ppm of iron sulphate when combinedly used with 50% tannery effluent. Organic matter was more with 10ppm of zinc sulphate, potassium sulphate and iron sulphate whereas CaCO3 was higher with 25 ppm of potassium sulphate and iron sulphate, when combinedly used with 50% tannery effluent. Chromium was higher with 10 ppm zinc sulphate, potassium sulphate and iron sulphate when combinedly used with 50% tannery effluent. The zinc was higher with 10 ppm of potassium sulphate, iron sulphate and 25 ppm of zinc sulphate while iron was found higher with 25 ppm of zinc sulphate, iron sulphate and 10 ppm of potassium sulphate when combinedly used with 50% tannery effluent. Potassium was found higher with 10 ppm of zinc sulphate, and 25 ppm of potassium sulphate, iron sulphate when combinedly used with 50% tannery effluent.

Keywords: Tannery effluent, Chromium, Protein, Sugars, Catalase, Peroxidase, Spinach, Organic Matter,

calcium carbonate, Zinc sulphate, Potassium sulphate and Iron sulphate

59

Res. Environ. Life Sci., 11 (11) 285-291 (2018)

Deforestation in Kerala over a quarter century (1993-2017) and the outbreak of Nipah Virus: an analysis using remote sensing technology and GIS approach

Beadanga Talukdar*, Ankit Sikarwar and Mayanka Ambade

International Institute for Population Sciences, Deonar Mumbai-400088, India

*Corresponding author e-mail: bedanga3@gmail.com

Paper received: 28.09.18; Revised received: 22.10.18; Paper Accepted: 25.10.18; Category: Original paper

 

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Abstract: Habitat destruction due to infrastructural, and developmental activities are primarily associated with outbreak of zoonotic infections. The habitat change negatively impacts ecological integrity and biodiversity by disrupting food web structure of “flying fox” fruit bat (pteropus). Squeeze in food recourses leads in nutritional and physiological stress among bats. That brings bats closer to human habitation and increases the chances of exposure to food near and transmitting it to humans. Henceforth the present study aims to analyse the extent of forest cover loss for the period from 1993 to 2017 in the four Nipah struck districts of Kerala. Which shall enhance our understanding of depletion in forest cover depletes functional richness of species and bats which shall have implications in outbreak of diseases in the ecological process. Data and methodology: The satellite data derived from the United States Geological Survey (USGS) in the form of Landsat Thematic Mapper-5 (TM5) from the years of 1993 and 2017 for the four districts of Kannur, Kozhikode, Malappuram, and Waynad in the state of Kerala, where recent outbreak of Nipah 2018 is used in the analysis. In the present study, Supervised Classification Scheme is applied to calculate the spatial change in forest cover over time. Moreover, the NDVI (Normalized Difference Vegetation Index) is calculated to find out the changes in the density and quality of forest which shall comprehend our knowledge on the susceptibility of bats to land use land cover change. The Nipah out-broke districts all together have experienced an extensive decline in forest cover (2,03,939, hectares) from 1993 to 2017. The loss is significant as about 27% of its forest has been lost in the area of interest. The Mean level of NVDI statistics has shifted to lower values indicating an alarming decline in dense vegetative cover over the years. The entire micro-region with spares vegetative cover suggests a change in habitat with reduced food resources for bats. The Nutritional and physiological stress pushed the vectors of the pathogen into human settlement resulting in the zoonotic outbreak.

Keywords: Deforestation, Habitat loss, NVDI, Nipah, Kerala

60

Res. Environ. Life Sci., 11 (11) 292-295 (2018)

Fungicidal management of powdery mildew disease of wheat in Jammu

Shahid Ahamad*1, Jag Paul Sharma1, M.K. Pandey2, B. Lal3, Satish K. Sharma1, and Amitesh Sharma1

1Directorate of Research, 2AICRP-Wheat, 3KVK, Reasi, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu (J&K), India

*Corresponding author e-mail: shahid_2056@yahoo.co.in

Paper received: 16.09.18; Revised received: 24.10.18; Paper Accepted: 29.10.18; Category: Original paper

 

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Abstract: Powdery mildew caused by Erysiphe graminis D.C. f.sp. tritici Marchal ranks third among the wheat diseases inflicting considerable losses in many parts of the world. In India, the disease is mainly confined to the northern and southern hills. The result revealed that all the treatments records significant reduction of disease incidence and increase yield. Two spray of Tilt 25 EC (0.1%) were recorded highest yield (44.57 q/ha), followed by one spray of Tilt 25 EC (42.07 q/ha) and Karathane @ 0.05% after 15 days interval (40.47 q/ha) respectively. While highest yield increased was found in case of two spray of Tilt 25 EC i.e.10.48 q/ha followed by one spray of Tilt 25 EC i.e.7.45q/ha and two spray of Karathane @ 0.05% i.e. 6.15 q/ha respectively. Whereas minimum disease severity was observed in case of two sprays of Tilt (0.01%) at 15 days interval was 0.27 %, followed by one spray of Tilt 25 EC i.e. 2.51% and two spray of Karathane 350 EC @ 0.05 i.e. 4.07 %, respectively. It was observed that propiconazole sprayed field remained free from the disease.

Keywords: Wheat, Triticum aestivum, Erysiphe graminis f.sp. tritici, Powdery mildew

61

Res. Environ. Life Sci., 11 (11) 296-299 (2018)

Effect of salinity on shoot fresh and dry mass in soybean (Glycine max L.)

 

Naheed Siddiqui*

Rajat P.G. College, Kamta, India

*Corresponding author e-mail: drnaheedlohani@gmail.com

Paper received: 06.08.18; Revised received: 11.10.18; Paper Accepted: 17.10.18; Category: Original paper

 

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Abstract: The soybean agro-industries play a vital role in strengthening the national economy as it provide livelihood to people in millions. The soy meal has the potential to ensure food and nutrition security in the country, particularly among the weaker section of the society. As salinization of soils became an important problem world-wide in determination of responses of cultivated plants, which differ in salt tolerance is an important phenomenon in distinguishing plant salinity relations. Salinization of agriculture arced as due to intense practices and irrigation is an important feature limiting crop yield and productivity. The impact of salinity irrigation on plant biomass was also evaluated in relation to levels and durations of the salinity. The data shown in paper favored inability in parallel with the higher levels of salinity in relation to retention of shoot fresh mass and shoot dry mass. The loss in shoot mass as shown by PS-1241 ranged from 22-27% in comparison to 41-47% incase used lower (50 mM) and higher (150 mM) levels of salinity in response to treatment duration (60-105 days). Almost similar losses have also been show with other two cultivars of soybean.

Keywords: Salinity, Soybeans, Shoot and root mass, NaCl, saline soil

62

Res. Environ. Life Sci., 11 (11) 300-302 (2018)

Effect of salinity stress on maize and gram with recovery treatment of ascorbate

Manoj Kumar Soni

Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow, India

*Corresponding author e-mail: bedanga3@gmail.com

Paper received: 15.08.18; Revised received: 16.10.18; Paper Accepted: 19.10.18; Category: Original paper

 

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Abstract: Salinity caused the damage, the chlorophyll contents (total, a and b) and a/b ratio showed a decline with increasing concentration of NaCl. When the total chlorophyll content and a/b ratio were 3.061 and 1.37 respectively in control, the addition of 50 mM NaCl decreased them to 3.000 and 1.32 respectively in maize plant. However, the antioxidant pigments-pheophytin ((total, a & b) and carotenoid contents showed increase with increasing stress by NaCl. At 200 mM NaCl concentration total pheophytin and carotenoid contents were found maximum (4.123 and 1.178 respectively) while at 50 mM NaCl they were a bit lower (3.919 and 1.040 respectively). The total pheophytin and carotenoid contents were less in ascorbate treated plants (4.045 and 1.139 respectively) than ascorbate untreated 200 mM NaCl plants (4.123 and 1.178) showing that ascorbate treated plants were less affected by damage caused by NaCl. In gram plants chlorophyll contents (total, a & b) showed a decline with increasing concentration of NaCl. The total chlorophyll contents were 2.399 in control. The addition of 50 mM NaCl decreased them to 2.300. However, the antioxidant pigments- pheophytin ((total, a & b) and carotenoid contents showed an increase with increasing salinity stress by increasing NaCl concentration. At 200 mM NaCl concentration total pheophytin and carotenoid contents were maximum (4.041 and 1.332 respectively). Application of ascorbate led to an increase in chlorophyll contents while decrease in pheophytin and carotenoid contents as compared to ascorbate untreated plants provided with same concentration of NaCl e.g. plants treated with ascorbate and 200 mM NaCl showed higher chlorophyll content (2.140) than plants treated with 200 mM NaCl alone (2.014).suggests a change in habitat with reduced food resources for bats. The Nutritional and physiological stress pushed the vectors of the pathogen into human settlement resulting in the zoonotic outbreak.

Keywords: Maize, Gram, Salinity, Ascorbic acid, NaCl, Pigments

63

Res. Environ. Life Sci., 11 (11) 303-308 (2018)

Perspectives on sustainable strategies for control of water pollution

Mayank Pushkar*1, Ratna Katiyar1 and H.P.S Rathore2

Department of Environmemtal Science, Univeristy of Lucknow, Licknow-226001, India; 2CPCB, Delhi, India

*Corresponding author e-mail: mayank8886@gmail.com

Paper received: 08.08.18; Revised received: 17.10.18; Paper Accepted: 21.10.18; Category: Review Article

 

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Abstract: In the recent years, there has been a remarkable growth of interest in environmental issues and sustainability. Associated with this growth several national and international agencies improve the understanding between environment and development, for which sustainable strategies for any kind of development is an important example. Environmental pollution is considered as unfavorable alternation in the surrounding caused by anthropogenic activities, changes in the living standards and energy use pattern, population growth. Environmental pollution is of global concern and both developing and developed countries have given wide attention towards its consequences. Deterioration in environmental quality as a result of pollution is demonstrated by loss of biological diversity, release of harmful chemicals in the ambient environment and their accumulation in the organisms and food grains etc. Among various dimensions of environment water is considered as critical resource for sustaining various life forms on the planet. Rapid industrialization, uncontrolled rise in population, urban sprawl have significantly contributed in the depletion of water quality. This paper reveals about the Perspectives on Sustainable Strategies for Control of Water Pollution.

Keywords: Perspectives, Sustainable Strategies, and Water Pollution

64

Res. Environ. Life Sci., 11 (12) 309-311 (2018)

Assessment of protein content in the some agro waste materials

Madhu Prakash Srivastava

Mamta Girls Degree College, Barabanki, India

*Corresponding author e-mail: madhusrivastava2010@gmail.com

Paper received: 02.10.18; Revised received: 19.11.18; Paper Accepted: 21.11.18; Category: Original paper

 

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Abstract: Agricultural products can contain substantial amounts of proteins. A number of proteins both derived from plants and animals, have been produced commercially for a long time. These proteins, such as potato proteins, proteins from cereals, soybeans and milk, are being used in food and feed, as well as in various non-food or technological applications. In the case of Bio-fertilizer enhances organic materials availability to plant more than an ordinary organic fertilizer. This is an environmentally friendly biotechnological approach also offers as an alternative to hazardous chemical fertilizers. In this present investigation to Assessment of Protein Content in the some agro waste materials were evaluated. In this regard after inoculation with Trichoderma LUNS1, the changes in chemical profile were observed in all the substrates used for mass multiplication of Trichoderma LUNS1 over uninoculated control. Combination of wheat bran and rice bran has shown the highest content of protein. The increase in Trichoderma LUNS1 inoculated wheat bran + rice bran was 73.777 over the uninoculated wheat bran+ rice bran. So, Agricultural activities and food industry generate considerable quantities of wastes which are rich in protein and could constitute new materials for value added products.

Keywords: Agricultural activities, Protein Contact, Agro Waste Material, Trichoderma LUNS1

65

Res. Environ. Life Sci., 11 (12) 312-316 (2018)

Inducing early flowering trait along with drought tolerance in Improved White Ponni through introgression of qDTY3.1 from APO

Madhu Prakash Srivastava, Shamini, K., Muthukumar, M., Raveendran, M., Sudhakar, V., and John Joel

Tamil Nadu Agricultural University, Coimbatore-641 003, India

*Corresponding author e-mail: jnjoel@gmail.com

Paper received: 19.07.18; Revised received: 22.11.18; Paper Accepted: 27.11.18; Category: Original paper

 

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Abstract: A major breeding objective in rice breeding programs is developing early maturing rice varieties that can reduce crop duration and enable 2-3 crops per year. Combining this early maturation trait along with drought tolerance is possible in improving stress tolerance in rice varieties through marker assisted introgression of mega effect QTLs from Apo, an upland drought tolerant rice variety. In this context, the present study was conducted for introgression of drought tolerance with early maturation in the genetic background of Improved White Ponni (IWP), a drought susceptible, fine grain type, popularly cultivated variety in Tamil Nadu, Indiathrough marker assisted QTL introgression from Apo, harboring large effect QTLs controlling grain yield under drought stress (viz., qDTY 1.1, qDTY 2.1, qDTY 3.1).Two superior lines of BC2F3generation of IWP x Apo population (BILs) with 2 QTL combinations under homozygous conditions;i) Class A (qDTY 1.1 +qDTY 3.1): BIL # 4-2-2 in, and ii) Class B (qDTY2.1+ qDTY 3.1): BIL # 4-7-4were selected by foreground selection using SSR markers linked with target QTLs and background selection with50genome wide polymorphic SSR markers.Both these BILsharbored one QTL in common, i.e., qDTY3.1 that is responsible for conferring early flowering trait. These BILs were evaluated for their phenotypic performance under drought stress conditions in the field which revealed that both the BILs performed better over the recipient parent IWP fortraits like spikelet fertility percentage,grain yield and plant height. Earliness was induced in both the BILs as the days to flowering was found to be advanced by 20-25 days earlier than IWP, which could be attributed to qDTY3.1. This study confirms that the introgression of qDTY3.1 could help in developing early maturing rice varieties.

Keywords: Near isogenic lines, Improved White Ponni, qDTY3.1, early maturation, days to flowering, drought tolerance, QTL introgression

66

Res. Environ. Life Sci., 11 (12) 317-321 (2018)

Effect of BA (Benzyladenine) during floral induction in cucumber

Yogendra Kumar Singh, Gopal Tiwari and Sandeep Kaur

Department of Botany, University of Lucknow, Lucknow-226001, India

*Corresponding author e-mail: yogi.251976@gmail.com

Paper received: 24.07.18; Revised received: 11.11.18; Paper Accepted: 16.11.18; Category: Original paper

 

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Abstract: The experiment show the effect of varying BA concentration and duration on the activity of two key enzymes of carbohydrate metabolism viz. sucrose synthase and acid invertase during floral induction in cucumber. During pre-flowering stage the sucrose synthase activity consistently decreased as compared to control with the increasing concentration of BA. It was 171.60 µmol/gm fresh weight in control and declined to 155.05, 147.60 and 138.27 µmol/gm fresh weight in plants exposed to 0.5, 5.0 and 50 µM BA for 24 hours respectively; the last value showing a significant difference with control. The increasing concentration and duration of BA treatment showed a cumulative effect on the activity of sucrose synthase as well. During flowering its activity was found to be 154.60 µmol/gm fresh weight in control, a marked decrease from the pre-flowering activity of 171.60. At pre-flowering acid invertase activity was 103.38 µmol/gm fresh weight in control which increased to 114.00 µmol/gram fresh weight when 0.5 µM BA was applied for 24 hours. The highest activity of acid invertase was recorded in plants treated with 50 µM BA for 72 hours (131.84 µmol/gm fresh weight) which was a significantly lower value than control. At the pre-flowering stage the activity of IAA oxidase increased as compared to control with the increasing concentration of BA. In control it was 0.604 µg IAA oxidized /gm fresh weight/hr at pre-flowering stage which increased to 0.618, 0.624 and 0.626 µg IAA oxidized /gm fresh weight/hr in plants given 0.5, 5.0 and 50 µM BA for 24 hours respectively. The increased duration of BA treatment also increased the IAA oxidase activity. At flowering the IAA oxidase activity was lower than that at pre-flowering stage in control as well as in treated plants. The maximum activity of IAA oxidase was recorded in plants treated with 50 µM BA for 72 hours (0.623 µg IAA oxidized /gm fresh weight/hr); a considerably significant value. In contrast to the activity of IAA oxidase, the activity of PPO as well as of peroxidase increased in both the conditions – when plants switched from pre-flowering stage to flowering stage and also when BA was applied, the concentration and duration again showing a cumulative effect. The highest peroxidase activity, just like that of PPO, was recorded in 50 µM/72 hours BA at both pre-flowering stage (22.48 DO.D./gm fresh weight) and flowering stage (28.08 DO.D./gm fresh weight).

Keywords: cucumber, (Cucumis sativus L.), Sucrose, Acid Invertase, IAA, Polyphenoloxidase, Peroxidase

67

Res. Environ. Life Sci., 11 (12) 322-324 (2018)

Estimation of median lethal concentration (LC50) and it’s criteria

Dharam Singh*1, Kamlesh K. Yadav2, Kamlesh Nath3 and S. P. Trivedi2

1Department of Environmental Science, I.B.S.B.T., C.S.J.M. University,

                                                  Kanpur-208002, India

2Department of Zoology, University of Lucknow, Lucknow-226001, India

3E.C.S., A.R.P. Ltd. Lucknow, India

*Corresponding author e-mail: ds_envi@rediffmail.com

Paper received: 04.08.18; Revised received: 16.11.18; Paper Accepted: 21.11.18; Category: Original paper

 

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Abstract: The median lethal concentration (LC50) is usually described as the concentration of chemical in the water that cause death to the 50% of the animals in a given population under a defined set of experimental condition. Acute lethality studies were essential for characterizing the toxic effects of chemicals. The method employed in the present investigation for the estimation of LC50 was Trimmed Spearman Karber Method. Several sets of experiments were designed to generate the raw data for the determination of LC50 after 96 hours of the exposure period. The 10 concentrations were selected in logarithmic ratio. In each experiment 10 fishes were subjected to the test chemical and a close watch kept over their behavioral activities. Fish mortality was recorded at a regular interval. The raw data so generated for the purpose of estimation of median lethal concentration of test chemical copper sulphate II were fed in software of Trimmed spearman Karber method. The values for different exposure period along with their 95% upper and lower confidence limits were obtained.

Keywords: Median lethal concentration (LC50), Trimmed Spearman Karber Method, copper sulphate, Toxicity test

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Res. Environ. Life Sci., 11 (12) 325-328 (2018)

Toxicity evaluation of linear alkyl Benzene Sulfonate (LAS) on liver of fishes

Abha Trivedi*1 and Kamlesh K. Yadav2

1Department of Animal Science M.J.P.Rohilkhand University, Bareilly-243006

1Department of Zoology, Government Degree College, Bakkha khera, Unnao-209801

*Corresponding author e-mail:  abha14sep@gmail.com

Paper received: 16.08.18 , Revised received: 11.11.18, Paper Accepted: 15.11.18, Category: Review Article

 

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Abstract: Contamination of aquatic resources with different kinds of pollutants has become a matter of concern over the past few decades. Uncontrolled discharge of domestic waste into water bodies leads to ecological risk from detergents that result into increased mortality rate of aquatic flora and fauna. Detergents are one of the most common pollutants found in all water bodies, entering through sewage outfalls. Linear Alkyl Benzene Sulfonate (LAS) is the most widely used anionic surfactant having enormous application in formulation of household cleaning products (dishwashing liquids, and laundry detergent), personal care goods, textile and food industries. The surfactants contribute as the major ingredient of a detergent used for cleaning purposes. Fishes are the main sufferer of detergent pollution as they acquire top position in aquatic food chain. Histopathological changes have widely been used as biomarkers in the assessment of the health of fishes exposed to contaminants. Liver is a very important organ as it is responsible for accumulation and biotransformation of xenobiotics in the fish and serve as warning signs of damage to fish health.

Keywords: Fish, Liver, LAS, Detergent

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Res. Environ. Life Sci., 11 (12) 329-332 (2018)

Application of remote sensing for evaluating

the wetland resources of Mohanlalganj area in Lucknow

Mujeeb Akhter*1, Noorul Huda2 and Kafeel Ahmad3

1GeologyDepartment, University of Lucknow, Lucknow-226007, India

2Department of Civil Engineering, LDC Institute of Technical Studies, Soraon, Allahabad-212502, India

3 Department of Civil Engineering, Jamia Millia Islamia, Jamia Nagar, New Delhi-110025, India

*Corresponding author e-mail:  mujeeballahabad321@gmail.com

Paper received: 07.07.18, Revised received: 15.11.18, Paper Accepted: 19.11.18, Category: Original Article

 

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Abstract: Remote sensing is the science of acquiring information, detecting, analyzing, and monitoring the physical characteristics of an area, water body, object or phenomenon without any physical contact with the target under study with the use of a geographical information system (GIS). This technique has potential applications in watershed management like water resource mapping and mapping of waterlogged areas. In this study we present the application of this technique for evaluating the wetland water resources of the Mohanlalganj area of Lucknow district.

Keywords: Remote sensing, GIS, wetlands, water resource

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Res. Environ. Life Sci., 11 (12) 333-337 (2018)

Use of wetland resources of Mohanlalganj administrative division for solving water crisis in urban areas of Lucknow: A model study on utilization of wetland resources as a remedy for water deficient areas

Mujeeb Akhter*1, Noorul Huda2 and Gauher Mahmood3

1GeologyDepartment, University of Lucknow, Lucknow-226007, India

2Department of Civil Engineering, LDC Institute of Technical Studies, Soraon, Allahabad-212502, India

3 Department of Civil Engineering, Jamia Millia Islamia, Jamia Nagar, New Delhi-110025, India

*Corresponding author e-mail:  mujeeballahabad321@gmail.com

Paper received: 09.07.18, Revised received: 03.11.18, Paper Accepted: 08.11.18, Category: Original Article

 

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Abstract: The present work deals with the utilization of excess available water present in the wetlands adjacent to city of Lucknow to address the problem of depletion of ground water and the consequent water scarcity in the urban areas of Lucknow. It was observed that the water level of pre- and post-monsoon period in the Mohanlalganj and Gosainganj areas is 0 to 2 m below ground level. As a result of the high water table large parts of Mohanlalganj administrative division are affected by the problem of water-logging. This study has been designed to solve the r problem of water scarcity in Lucknow city by transferring excess water from water logged areas of Mohanlalganj tehsil, thus also addressing the problem of water logging in   Mohanlalganj administrative division. The proposed study can serve as a potentially viable and sustainable solution for addressing the problem of water scarcity due to ground water depletion in an area by sourcing water from adjacent areas with surplus ground water resources.

Keywords: Wetland, ground water, water scarcity, water resources, Lucknow