RESEARCH IN ENVIRONMENT AND LIFE SCIENCES

Volume-12, Number-3, July, 2019

 

20.

Res. Environ. Life Sci., 12 (3) 89-91 (2019)

 

Effectiveness of variable growth mediums (Nitsch, Knop’s and Knudsen’s medium) on in vitro propagation and multiplication of Asterella wallichiana (Lehm. et  Lindenb.)

 

Mridula Maurya*

Botany Department, University of Lucknow, Lucknow 226007, India

 

*Corresponding author e-mail: mridulamaurya.mailbox@gmail.com

 

Paper received: 14.04.19, Revised received: 18.05.19

Paper Accepted: 24.05.19, Category: Original paper

 

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Abstract: The propagation and multiplication of Asterella wallichiana (Lehm. et Lindenb.) Pande et al. was carried out under controlled laboratory conditions to compare the effect of three growth mediums (Nitsch, Knop’s and Knudsen’s medium). Nitsch medium was used with three variations such as Nitsch medium, Nitsch medium (with NAA and KN) and Nitsch medium without agar. As compared to all types of growth mediums used, Nitsch medium (with NAA and KN) was observed to be most effective in multiplications and propagation of Asterella wallichiana liverwort and helpful in the conservation of biodiversity of bryophytes.

Keywords: Thalloid, Liverwort, in vitro, Propagation

21.

Res. Environ. Life Sci., 12 (3) 92-93 (2019)

 

Selection of suitable varieties of chickpea in dryfarmed area of Bundekhand for grain and vegetable

 

R.A. Singh*1, Shanker Singh1, Ram Prakash1, R.K. Singh2, Jitendra Singh1, and Asha Yadav1

1C.S. Azad  University of Agriculture and Technology, Kanpur (U.P.), India,  2K.V.K., Jalaun (U.P.) India

 

*Corresponding author e-mail: rasinghcsau@gmail.com

 

Paper received: 22.04.19, Revised received: 28.05.19

Paper Accepted: 02.06.19, Category: Original paper

 

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Abstract: The study was under taken during two consecutive winter season at Pilot Project of Watershed, rendhar, Jalaun under rainfed situation. The operational area of watershed typically represent soil, climate and socio-economic condition of Bundelkhand Region. The moisture availability period for crop growth is 122 days. The II and III land capability class soil, having poor fertility status. The eight varieties of chickpea i.e., Radhey, K-850, K 468, Awrodhi, type 3, H 208, Pusa 267 and Pusa 261 were tested under four levels of nitrogen and phosphorus @ control (N0P0), N5P20, N10P40 and N15P60 kg/ha. Among the test cultivars Radhey registered highest kernel yield of 21.00 q/ha. varieties K 850, K 468 and Awrodhi gave kernel yield of 20.50 q/ha, 20.41 q/ha and 20.11 q/ha, respectively, which were almost similar to the kernel yield of Radhey. The test cultivar Type 3, H 208, Pusa 267 and Pusa 261 gave kernel yield by 18.12 q/ha, 15.89 q/ha, 14.76 q/ha and 13.99 q/ha, respectively. The lowest kernel yield was reaped from cultivar Pusa 261. The grain yield of all the cultivars increased upto 15 kg N + 60 kg P2O5/ha over the lowest installments of nitrogen and phosphorus. For brown Chhola purpose cultivars K-850 proved superior.

Keywords: brown Chhola, Bundelkhand, Land capability, Moisture availability, Watershed technology

 

22.

Res. Environ. Life Sci., 12 (3) 94-96 (2019)

 

 

Effect of some pesticides on absolute values (MCV, MCH, MCHC) of Anabas testudineus (bloch)

 

Mamta Sinha*1 and  Renuka Sharan2

1Department of Zoology, G.B.M. College, Gaya, India

2Department of Zoology, College of Commerce, Patna, India

 

*Corresponding author e-mail: mamtasinha1956@gmail.com

 

Paper received: 18.03.19, Revised received: 11.05.19

Paper Accepted: 15.05.19, Category: Original paper

 

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Abstract: Anabas testudineus, a fresh water teleost when treated with three pesticides namely Metacid, Malathion (Organophosphate) and Lindane (Organochlorine) to their Lc50 doses for 24hrs, 48 hrs and 72hrsof exposure for analyzing the effect of toxicity of pesticides on absolute value (MCV, MCH, MCHC). The value of MCV in fishes treated with Malathion and Lindane was always higher than the control value in all the three duration of exposure. But in the case of Metacid treated fishes on 24hrs of exposure the MCV value was insignificantly lower than the control value but increased value was recorded in 48hrs and 72 hrs of exposure. The value of MCH in fishes treated with Metacid was lower than the control value on 24 hrs, 48 hrs and 72 hrs of exposure. The value of MCH is always higher than the control value, when treated in fishes with Malathion and lindane in all the three duration of exposures. The different degree of variation in the MCHC values was recorded in all the three concentration of Metacied, Malathion and Lindane. It was observed that the MCV value for Metacid of blood was 66.37mm3, 76.00mm3 and 76.63mm3 in fishes exposed to Lc50 concentration for 24hrs, 48hrs and 72 hrs respectively for metacid exposure showing a 6.5% fall in 24hrs, while 7% and 7.9% in 48 hrs and 72 hrs from the control value (70.99mm3). In the case of fishes exposed to the Malathion the MCV  value was 80.39mm3, 78.50mm3 and 81.17mm3  for 24 hrs, 48 hrs and 72 hrs respectively showing a rise of 13.2%, 10.5% and 14.3% from the control value (70.99mm3). In the case of Lindane, the MCV value was 79.35mm3, 82.12mm3 and 76.65mm3 for 24 hrs, 48 hrs and 72 hrs respectivley, showing a rise of 11.7%, 15.6% and 8% from the control value (70.99mm3). The MCH value decreased by 5.9%, 2% and 6.5% from control value (37.78 Pg), after 24 hrs, 48hrs and 72 hrs of exposure with Metacid. The value of MCH always rises by 8.1%, 10.4% and 19.8% from control value with Malathion. In Lindane the MCH value rises by 12.7%, 10.9% and 21.9% from control value when recorded. The MCHC value showed marginal increase in the fishes exposed to Lc50 dose for 24hrs (53.56%) while in an exposure to Lc50 dose for  48 hrs, and 72 hrs a fall in the MCHC value to 48.68% and 46.09% respectively exhibiting a fall of 8.53% and 13.39% from the control value (53.22%) with metacid. In the case of malathion a fall of 4.5% was recorded for 24 hrs, but in case of 48hrs a very less fall of 0.09% was recorded while in 72hrs an increase value of 4.79% from control value was recorded. In  Lindane for 24 hrs of Lc50 dose the MCHC value rise by0.8% while on the exposure of LC50 dose for 48hrs and 72hrs duration a decreasing  value was observed i.e. 4.1% and 0.07% from the control value respectively.

Keywords: Absolute value (MCV, MCH, MCHC) pesticide effect on Haematology, Anabas testudineus

23.

Res. Environ. Life Sci., 12 (3) 97-99 (2019)

 

A study on the nutritional status of school going children (10-12 years) of mid day meal programme in Allahabad district

 

Sunita Sharan*

SGNGDC, Lucknow, India

 

*Corresponding author e-mail: sunitasharan73@gmail.com

 

Paper received: 22.03.19, Revised received: 17.05.19

Paper Accepted: 19.05.19, Category: Original paper

 

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Abstract: The Mid day Meal Scheme is a school meal programme of the Government of India designed to improve the nutritional status of school-age children nationwide.  According to WHO malnutrition continue a major health problem in south East Asian region. Studies have reported a large section of children especially the urban area in India are suffering from varying grades of malnutrition. The results obtained after the survey conducted showed that the average height and weight of Mid- Day Meal beneficiaries girls were better than the boys. In boys mean daily intake of energy, calcium, vitamin A, b carotene and vitamin C were lower than the RDA and in girls, protein, calcium, vitamin A, b carotene and vitamin C were lower than the RDA. The nutrient content of Mid-Day Meal which was provided by the government schools were found is higher in macro nutrients like energy, protein, fat, carbohydrate and calcium.

Keywords: Mid day meal, nutrition, children, calcium, vitamins, protein,

 and b carotene

24.

Res. Environ. Life Sci., 12 (3) 100-104 (2019)

 

 

Assessment of soil and water characteristics in Barabanki district through survey and physico-chemical analysis and recommendation of suitable crops

 

Veer Pratap*, Akhand Pratap Singh and Y.K. Sharma

Maharishi University of Information Technology, Lucknow

 

*Corresponding author e-mail: veerpratap0446@gmail.com

 

Paper received: 02.03.19, Revised received: 18.05.19

Paper Accepted: 21.05.19, Category: Original paper

 

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Abstract: To explore the diversified crop pattern in barabanki district, a survey was made and sampling was done in different blocks and villages to study the physico-chemical properties of soil and water. Among the crop pattern it was found that most of the cereal crops including the millets like jower and bajra, common pulses and the cash crops like sugarcane and potatoes are grown. Among the physico-chemical properties of soil and water, pH, EC and TDS were studied. The minimum pH of water was observed 7.4 maximum pH of water was observed 8.5, minimum EC was observed 1.08 and maximum EC was observed 2.98. The minimum pH of soil was observed 7.2 and maximum pH of soil was observed 9.9, minimum EC was observed 0.42 and maximum EC was observed 3.80.

Keywords: Jower, Bajra, Pulses, pH, EC, TDS

25.

Res. Environ. Life Sci., 12 (3) 105-111 (2019)

 

Effect of different concentrations cobalt on the growth and certain metabolic parameters of Oryza sativa

 

Akash Vikram and Tulika Johri*

Department of Botany, Lucknow University, India

 

*Corresponding author e-mail: akashvikram05@gmail.com

 

Paper received: 01.04.19, Revised received: 26.05.19

Paper Accepted: 30.05.19, Category: Original paper

 

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Abstract: An experiment was carried out to study the effect of different doses of Cobalt viz. 0.25 mM, 0.5 mM and 1 mM of Co along with control on the growth and certain metabolic parameters in Oryza sativa plants. It was observed during the experiment that at increasing doses of Co, all the growth parameters i.e, plant height, fresh and dry weight and water content was found to be decreased at increasing doses of Co. Different doses of Co was also found to be toxic for studied metabolic parameters i.e concentration of photosynthetic pigments, activity of antioxidative enzymes i.e, catalase, peroxidase and contents of proteins and sugars. The only exception was that in case of catalase activity where an enhancement was observed at lowest dose of 0.25mM Co. However, further enhancement of dose of same heavy metal caused decrease in the activity of this enzyme also.

Keywords: Cobalt, Chlorophyll, Catalase, Peroxidase, Sugar, Protein