RESEARCH IN ENVIRONMENT AND LIFE SCIENCES
Volume-12, Number-4, October, 2019
(A Multidisciplinary, Peer reviewed / Referred Research Journal
Concerned with Environment and Life Sciences)
(p-ISSN: 0974-4908)
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Res.
Environ. Life Sci., 12 (4) 112-114
(2019) Biochemical alteration in plasma protein content in blood of Anabas
testudineus (bloch)
due to effect of pesticides Mamta Sinha*1 and Renuka Sharan2 1Department of Zoology, G.B.M. College, Gaya, India 2Department of
Zoology, College of Commerce, Patna, India *Corresponding author e-mail:
mamtasinha1967@gmail.com Paper received: 23.02.19, Revised received:
19.05.19 Paper
Accepted:
23.05.19, Category: Original paper |
Abstract:
In
the present work an attempt has been made to study the alteration in the
plasma protein level in a fresh water air breathing fish, Anabas testudineus (bloch) exposed
to three pesticides namely metacid, malathion, and Lindane under 24, 48 and 72 hours of exposure
period. The treatment of above mentioned pesticides cause a continuous fall
in the plasma protein. Depletion in the plasma protein level was significant
in longer duration of exposure in all the three formulation of pesticides.
The plasma protein level in control fishes range between 8.67 to 9.27 mg 100 ml -1.
In the case of exposure to metacid for 24, 48 and
72 hours to LC 50 dose of pesticide the level of plasma protein
recorded was 8.83 mg 100 ml -1 (4.47 % of fall), 8.45 mg 100 ml -1 (8.8% fall) and
7.92 mg 100 ml -1 (14% fall) less than the control value. In the
case of malathion for 24,48 and 72 hours to the LC 50
dose of pesticide the level of plasma protein recorded was 8.04 mg 100 ml -1
(9 % fall), 7.62 mg 100 ml -1 (14% fall) and 7.13 mg 100 ml -1 (19% fall)
less than from the control value.While in the case
of lindane for 24, 48 and 72 hours to LC50 dose of pesticide ,the level of plasma
protein recorded was 7.68 mg 100 ml -1
(11 % fall), 7.35 mg 100 ml -1 ( 15% fall) and 6.85 mg 100 ml -1
(21% fall) from control value.The required
quantity of metacid to constitute LC50
dose for 24, 48 and 72 hours of exposure was 19.28 mg 100 ml -1,
13.18 mg 100ml-1. and 9.12 mg 100 ml -1 respectively. While
those of malathion and Lindane
was 16.22 mg
100 ml -1, 9.88 mg 100 ml -1, 8.91 mg 100 ml -1
and 12.16 mg 100 ml -1, 8.92 mg 100 ml -1 and 7.24 mg
100 ml -1 Respectively. The safe concentration of metacid was 8.56 mg 100 ml -1, while the
quantity of malathion for safe concentration was
7.97 mg 100 ml -1 and the quantity of lindane
for safe concentration was 5.02 mg 100 ml -1. Keywords:
Anabas
testudineous, Metacid, Malathion, and Lindane
pesticide |
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Res.
Environ. Life Sci., 12 (4) 115-119
(2019) Response of Vigna sinesis on application of different concentrations of
biopesticide and chemical pesticide Tulika Johri* and Akash Vikram Department
of Botany, Lucknow University, India *Corresponding author e-mail:
tulikajohri@gmail.com Paper received: 01.04.19, Revised received:
26.05.19 Paper
Accepted:
29.05.19, Category: Original paper |
Abstract:
The
effect of both bio-pesticide (Neem oil) and
chemical pesticide (cypermethrin) in different
concentrations viz. 0.2%, 0.4%, 0.8% and 1.6% were investigated on cowpea
plants. It was observed that on increasing the concentration of both
bio-pesticide and chemical pesticide upto 0.4%
plant height was found to be increased. However, this increase upto same concentration in case of chemical pesticide was
non-significant. At increasing doses of both bio and
chemical pesticides, number of leaves per plant, fresh weight and dry weight
per plant were significantly decreased. The same trend was observed in the
biomass yield of the crop. The water content per plant increased at 0.2%
concentration of both bio and chemical pesticides but further it was
decreased on increasing the concentration of both pesticides. However, such
decrease was higher in plants treated with chemical pesticide as compared to biopesticide. With regard to metabolic parameters,
concentration of photosynthetic pigment in general was found to be decreased
at higher concentration of both bio and chemical pesticides. Activity of
enzyme catalase was found to be significantly
increased at 0.2% and 0.4%
of both bio and chemical pesticides. However the enzyme peroxidase was significantly decreased beyond the
concentration of 0.4% of bio pesticide and beyond 0.2% of chemical pesticide.
Concentartion of both protein and sugar were found
to be decreased at increasing concentration of both chemical and bio
pesticides. Keywords
: Biopesticide (neem oil),
Chemical pesticide (cypermethrin), Chlorophyll, Catalase, Carbohydrate |
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Res.
Environ. Life Sci., 12 (4) 120-124
(2019) Effect of growth regulators (BA at seed germination and BA and
IAA at flowering stage) on activity of sucrose synthase
and acid invertase during floral Induction in
cucumber Yogendra Kumar Singh* Department
of Botany, K.S.Saket P.G. College, Dr. Ram Manohar Lohia Avadh University, Ayodhya,
India *Corresponding
author e-mail: yogi.251976@gmail.com Paper received: 11.04.19, Revised received:
27.05.19 Paper
Accepted:
28.05.19, Category: Original paper |
Abstract:
Flowers
represent specialized structures of a reproductive phase of shoot meristem activity. The conversion of the meristem from a vegetative to reproductive function and
the development of flowers involve direction by environmental factors,
sequential expression of integrated classes of genes, and numerous hormones.
Application of BA showed a significant reduction over control in plants given
50 µM BA at seed germination stage while no growth regulator was sprayed at
flowering (sucrose synthase activity 103.22 µmol/gm
fresh weight). Application of BA or IAA at flowering time further lowered the
activity of the enzyme with IAA causing a greater reduction than BA. The
occurrence of flowering caused a reduction in the activity of sucrose synthase in all the treatments including control. The
activity of acid invertase, however, showed a trend
just opposite to that of sucrose synthase. Thus,
while application of increasing concentration of BA at seed germination stage
caused a gradual increase in the activity of acid invertase
during pre-flowering stage, treatment of BA or IAA at the time of flowering
further increased the activity of the enzyme with IAA showing a greater
enhancing effect. Application of BA at seed germination stage and of IAA or
BA at flowering stage showed same effect as observed during pre-flowering
stage. Keywords: Activity of sucrose
synthase, acid invertase,
BA, IAA, flowering, seed germination |
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Exclusively Online Res.
Environ. Life Sci., 12 (4) 125-127
(2019) Adaptive measures of Crotalaria burhia
buch. ham. in arid
environment of Indian desert B.S.V. Prakash Department of Botany, Lucknow Christian Degree College, Lucknow,
India *Corresponding
author e-mail:
drbsvprakash@gmail.com Paper received: 19.03.19; Revised received:
18.07.19 Paper
Accepted:
23.07.19; Category: Original paper |
Abstract:
In
order to survive, plants must adapt to soil moisture stress, dryness of air andhigh atmospheric temperature associated with intense
solar radiations in the Indiandesert.
Differentiation of a species into a number of morphologically distinct
populations is one such adaptation in all extreme environmental conditions.
The presentstudy relates to such adaptation
displayed by Crotalaria burhia Buch. Ham, in Indianarid
tracts. Two distinctly variable populations of C. burhia
were observed in two different habitats, viz. open places (sand dune and
abandoned fields) and shady and protected sandy plains.Morphological
variations in root, stem, leaf, etc, were recorded at various growth stages.
Based on the distinct morphological variations, C. burhia.
populations have been classified as
‘erect-bushy’(EB) and ‘sub-erect
spreading’ (SS) forms, populations of EB form are considered to be drought
tolerant whereas the populations of SS form are drought escaping. Keywords: Crotalaria
burhia, morphological variations,
Adaptive measures |
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Exclusively Online Res.
Environ. Life Sci., 12 (4) 128-131
(2019) Molecular profiling of (Amorphophallus
paeoniifolius) varieties elephant foot yam Kshitij Kumar*, Shashi Devi and K.N. Singh Department
of Plant Molecular Biology & Genetic Engineering, Narendra
Deva University of
Agriculture & Technology Narendra Nagar
(Kumarganj), Faizabad,
U.P., India *Corresponding
author e-mail: Kshitijkumar2010@gmail.com Paper received: 15.05.19; Revised received:
21.10.19 Paper
Accepted:
26.10.19; Category: Original paper |
Abstract:
The
genus Amorphophallus paeoniifolius,
a tuberous herb belonging to the family Araceae
and a paleotropical aroid comprising of more than
200 species. It isone of the true marvels of nature
and a true treasure of variation. Amorphophallus (elephant foot yam) is an herbaceous
edible aroid crop which belongs to the family Araceae.
The study was undertaken to screen and obtained polymorphic bands with the
efficient EST-SSR primer that could differentiate a set of nine elephant foot
yam genotypes. Total nine Amorphophallus
paeoniifolius varieties (NDA 45, NDA 49, NDA
52, NDA 55, NDA 64, NDA 44, NDA 65, NDA 9 and Gajendra)
were screened with 11 EST-SSR primers and obtained polymorphic bands
with primers Amor 34 (190 bp) and Amor 46
(155 bp) and monomorphic
band with primers Amor 22, (160 bp) Amor 33
(170 bp) and Amor 36 (155 bp). Cluster analysis of combined banding pattern of EST-SSR
primers. The EST-SSR markers and protein analysis under this
investigation will facilitate further studies in linkage mapping, genetic
diversity, germplasm characterization and marker
assisted selection in Amorphophallus paeoniifolius. They could also be applied to the
genetic researches in other species and genera because of their high
transferability. Keywords: Elephant foot yam,
molecular profiling, Amorphophallus paeoniifolius |