RESEARCH IN ENVIRONMENT AND
LIFE SCIENCES
Volume-1, Number-3, August-2008
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23. |
Review Paper Res. Environ. Life Sci., 1(3) 81-90 (2008) Role of differentially
expressed ripening related genes and promoters in banana fruit: Identified by
mRNA DDRT-PCR Sudhakar Srivastava and Sanjay Mohan Gupta* Plant Molecular Biology and
Genetic Engineering Lab., Defence
Agricultural Research Laboratory, DRDO, Haldwani-263139, *e-mail: smg555@rediffmail.com |
Abstract: It is now becoming increasingly evident that
plants have a tight regulation of gene expression during fruit ripening which
is brought about through differential expression of hundreds of genes. This
review deals with the identification of several ripening related and ethylene
induced/suppressed genes in banana pulp using mRNA differential display
reverse transcription(DDRT-PCR). It also highlights
recent developments in identifying and assigning role to various ripening
related genes and promoters during ripening that may help to better
understand these phenomena at the molecular level and identify candidate
genes for genetic manipulation to improve the postharvest life of banana
fruits. Key words: Fruit ripening,Differential
gene expression,Banana,Ripening related gene and promoters,DDRT-PCR,Ethylene signalling |
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24. |
Original Paper Res. Environ. Life Sci., 1(3) 91-94 (2008) Effect of chromium and
tannery effluent toxicity on metabolism and growth in cowpea (Vigna sinensis L.
Saviex Hassk)
seedling Kamlesh Nath*, Dharam
Singh, Shilpa Shyam and Yogesh
Kumar Sharma Laboratory of Environment Sciences,Department of Botany, *e-mail: nathkamlesh@rediffmail.com |
Abstract: The petridish experiment was performed using
different dilution levels of treated tannery effluent (10, 20, 40, 60, 80 and
100 %) and their corresponding concentration of chromium (VI) (2, 10, 25 and
50 ppm )
to know the effect on seed germination and seedling growth in cowpea (Vigna sinensis L.
Saviex Hassk).
The germination percentage decreased with increase in concentration of
tannery effluent and chromium (VI). The different concentrations of tannery
effluent and chromium (VI) showed reduction in seedling growth (plumule
length, radicle length, number of lateral roots, fresh weight, dry weight and
moisture %) and pigments (chlorophyll, pheophytin and carotenoids) with increase
in concentration. Amylase activity and total sugar content also decreased,
while catalase and peroxidase activities increased with increase in
concentration. The lower concentration of tannery effluent dilution (10 and
20%) and chromium (VI) (2 and 10 ppm) showed
significant increase in total protein content, and decrease at higher
concentrations. Key words: Tannery effluent, Chromium
(VI), Amylase, Catalase, Peroxidase, Total sugar,Total
protein |
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25. |
Original Paper Res. Environ. Life Sci., 1(3) 95-98 (2008) Accumulation of heavy metals (Pb, Mn, Fe, Cd,
Cr, Zn and Cu) in road side trees Shilpa
Shyam*, Kamlesh Nath and Dharam Singh Laboratory of Environmental Sciences, Department of Botany,University of *e-mail: shilpa_lko@yahoo.com |
Abstract: Heavy metal contamination of urban air, soil and
vegetations has become a serious environmental problem. Metal accumulation in
urban areas is related to traffic. It has been found that petrol and diesel
driven vehicles emit heavy metals alongwith
particulate metter in to the urban environment as a
result of combustion of fuel. Heavy metal concentration was analysed in ashok (Polyalthia longifolia
Thw.) and peepal (Ficus religiosa ALinn.) leaves in summer, monsoon and winter season.The metal uptake by ashok
and peepal plant was found in the order of Fe<Pb<Zn<Cu<Mn<Cr<Cd and Fe<Pb<Zn<Mn<Cu<Cr<Cd
respectively. Analysis of data reveals that the accumulation of heavy metal
was higher in monsoon season and minimum in summer in case of most heavy
metals. The accumulation of heavy metals was found maximum in industrial
areas and minimum in residential areas. The cadmium and chromium was found
below the detectable limit in some cases. Data reveals that ashok plant has good metal accumulation capability than peepal plant. Key words: Accumulation, Heavy metals, Pb, Mn, Fe, Cd,
Cr, Zn and Cu, Air pollution |
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26. |
Original Paper Res. Environ. Life Sci., 1(3) 99-102 (2008) A Histopathological
study to evaluate hepatotoxic effect of chloremphenicol, zidovudine and
RISUG in albino rats Jyotsna*1,2, V. Gupta1 and R.K. Singh2 1Department of Zoology, 2Division of Toxicology,
C.D.R.I., *e-mail: jyotsnagaur@yahoo.com |
Abstract:Many drugs and chemicals causes disruption of its structural
integrity may inhibit physical, biochemical activities and liver diseases.
The liver is important organs in the body because of their involvement in
biotransformation and excretion of xenobiotic
respectively. Liver is a main storage and metabolizing centers of toxicants
in the animals. The aim of present study was to evaluate the toxic effect of
three drugs on liver, including antibacterial chloramphenicol,
anti-aids azidothymidine (zidovudine)
andmale infertility RISUG(reverse
inhibition of sperm under guaidence) drugsin Charles Fosterstrain of
albino rats. Chloramphenical,zidovudine were
administered orally to rats at the dose levels of 560, 240 mg kg-1
body weight respectively for 5 days,while RISUG,
0.5mg/ 0.03ml DMSO were implanted in each vas deferens of rat. Significant histopathological degeneration changes, swelling of hepatocytes were observed due to chloramphenicol
and zidovudine but no any cell damages were
observed in RISUG treated rats. Key word: Histopathology, Albino rats, Chloramphenicol,
Zidovudine and RISUG |
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27. |
Original Paper Res. Environ. Life Sci., 1(3) 103-108 (2008) Bioaccumulation
of heavy metal in crops irrigated with secondary treated sewage waste water
in surrounding villages of Abhishek Mukherjee* and Virendra Kumar Mishra Department
of Botany, *e-mail: mukherjeeda2003@yahoo.co.in |
Abstract: The contamination of agricultural products with
heavy metals resulting from waste water irrigation has become an important
concern throughout the world due to potential adverse effects of heavy metals
on human health. The present study was conducted to investigate the level of
heavy metal in irrigation water, soil and some vegetables like spinach and
cauliflower grown in the adjacent areas of sewage treatment plant (STP).
People in this area utilize treated sewage for irrigation for the last two
decades. The field study was conducted at three sites in the adjacent areas
of Bhagwanpur (STP), 10 MLD, Varanasi, Samples of
irrigation water, soil and edible portion of palak
(Spinach oleraceae) and cauliflower (Brassica oleracea
var. botrytis) were collected during summer and winter season and analyzed
for Pb, Cu., Zn, Cd and
Cr. The data generated show elevated level of some heavy metals in all
environmental media, suggesting a definite adverse impact on the
environmental quality of the disposal area. Heavy metals in irrigation water
were higher than the internationally recommended (WHO) maximum permissible
limit set for agricultural use for all heavy metals. Similarly the heavy
metal concentration in soil below the Indian standard for all heavy metals,
but the maximum value of Cr recoded during summer was higher than the standard
(13.95 mg kg-1). However in edible portion of spinach Cd, Cu and Cr concentration (5.93, 28.15 and 12.02 mg kg-1respectively,
in Madarva) was higher than the permissible limits
of Indian standard while in the edible portion of cauliflower Cr, and Cd (5.95 and 1.96 mg kg-1 respectively, in Madarva) were higher than the permissible limits. The
study revealed highly significant variation (p> 0.005) among different
sites. There was positive and significant correlation between heavy metal
concentration in secondary treated sewage and soil. This correlation was also
significant between metal concentration in treated sewage and concentration
in different plant parts. The study points to the fact that the use of
treated and untreated waste water for irrigation has increased the
contamination of Cd, Cu and Crin
the edible portion of vegetables causing potential health risk in the long
term from this practice. Key word: Heavy metal, Seavage waste water,
Cadmium, Copper, Chromium, Zinc, Lead, Bioaccumulation |
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28. |
Original Paper Res. Environ. Life Sci., 1(3) 109-112 (2008) Accumulation of heavy metals (Cd,
Cr, Ni, Co, Cu and Fe) in various parts of Zea
mays treated with asbestos effluent Amar Nath Giri*1, Aditya Verma2, Shiv 1Environment
Management System, NFCL, Nagarjuna road, 2Department
of Botany, *e-mail:
goswami818@yahoo.com, amarnathgiri@nagarjunagroup.com |
Abstract:The treated asbestos effluent was collected from asbestos industry.
Different concentrations i.e. control (0%), 20, 40, 60, 80 and 100% were
prepared from asbestos effluent. Maize (Zea
mays) was grown in sand pot culture. All metals
(Cr, Cd, Cu, Ni, Fe and Co) concentration was
increased with increasing concentration of treated effluent of asbestos
industry in tissues of root, stem and leaves. The heavy metals concentration
was significantly increased in root on 40, 60, 80 and 100% of concentration
of asbestos effluent. The plant tissue analysis in our experiment shows
higher accumulation of heavy metals in different plant parts with the
application of asbestos effluent, although the level of accumulation varied
from part to part. The higher accumulation of heavy metals in roots than
aerial parts can be explained as heavy metals remains bound in the roots that
is why did translocation from roots to leaves. Key words: Asbestos effluent, Chromium,
Cadmium, Copper, Nickel, Iron and Cobalt |
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29. |
Original Paper Res. Environ. Life Sci., 1(3) 113-116 (2008) Feeding
practices and dietary pattern of young children in rural area
Neelam Kumari* and Ajai Goswami *e-mail: neelamkumari080@gmail.com |
Abstract: Children below three years of age constitute a
little over 15% of our total population. They undoubtedly are most crucial
segment of our population due not only to their sheer number but also because
during these formative years foundations are laid for adult life. Moreover
the quality of our future human resource is going to be determined largely by
these children. Nutrition is a major factor in bringing out the maximum
potentiality that one is endowed with both physically and mentally. The
infants and pre-school children are extremely vulnerable. A study was
conducted in the western skirt of Key words: Children, Breast feeding,
Nutrition |
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30. |
Original Paper Res. Environ. Life Sci., 1(3) 117-118 (2008) Raising
of virus resistance inducing activity of Clerodendrum
aculeatum leaf protein using bio-enhancer of
biological origin Ahmad Najam*1, L.P. Awasthi2
and H.N.Verma1 1Plant
Virology Laboratory, Botany Department, 2Department
of Plant Pathology, *e-mail: najam_lko@yahoo.com |
Abstract: In the present investigation, attempts were made
to enhance the virus resistance inducing activity of C. aculeatum leaf extract protein in combination with
certain bio-enhancer of biological origin. Maximum percent inhibition was
reported at 1:5 dilution in all the cases whether CA-SRIP used alone or
CA-SRIP+BE (5% and 10%) combination .CA-SRIP+BE (5%) showed better response
than CA-SRIP+BE (10%). Key words: Clerodendrum aculeatum (CA), Systemic resistance inducing
protein (SRIP), Cyamopsis tetragonoloba, Sunnhemp
rosette virus (SHRV), Bioenhancer |
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31. |
Original Paper Res. Environ. Life Sci., 1(3) 119-122 (2008) Enhancement of efficacy in
management and control of yellow leaf curl viral disease in tomato (Lycopersicon esculentum
Mill.) using a herbal product GUJCO VIRCON Yogesh K. Sharma*, Priyanka Sharma and Savitri Singh Department of Botany, *e-mail:
yogesh_s26@yahoo.com |
Abstract: In a farmer field cum laboratory experiment the
efficacy of a herbal preparation GUJCO VIRCON regarding
management and control of the tomato yellow leaf curl virus (TYLCV) disease,
spreading through the vector whitefly - Bemisia
tabaci, was tested in sub-tropical climatic conditions
of Key words: TYLCV, GUJCO VIRCON, Bemisia
tabaci fruit yield |
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32. |
Original Paper Res. Environ. Life Sci., 1(3) 123-128 (2008) Qualitative assessment of municipal and ground water of Shashank Shekhar Mishra*
Ajai Mishra and Reetanjali Singh Centre of Advanced Study in
Geology, *e-mail: enviroshashank@rediffmail.com |
Abstract: The study was performed to know the status of
water quality of Key words:
Physico-chemical, Bacteriological properties, Heavy metals, Water |
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33. |
Original Paper Res. Environ. Life Sci., 1(3) 129-133 (2008) Study of temperature stress on seed germination, seedling
growth and biochemical parameters and recovery of damage by using Ascorbate Manoj Kumar Soni* Central Institute of
Sub-Horticulture (CISH), Rehmankeda, *e-mail: manojsoni_lko@yahoo.com |
Abstract:Present experiment was performed in laboratory at different temperature
levels (35 and 40oC) on maize and gram (for 7 and 14 days of
exposure). The seed germination was decrease with increase of temperature in
both seeds. In one and two week old seedlings of maize and gram were observed
for seed germination, morphological (plumule length, radical length, number
of lateral roots) and physiological (fresh wt., dry wt. and moisture %)
parameters. The pigment, amylase, protein and sugar contents were also
analyzed. The pigment, amylase, protein and sugar contents were decrease with
increase of temperature, although catalase and peroxidase activity were
increase. After one week ascorbate (50 mM) was
added in petridishes for the recovery purpose. This ascorbate application
promote the plumule length, radical length, number of lateral roots, fresh
wt. dry wt. and moisture (%), pigment, protein and sugar content, but
catalase and peroxidase activity decreases. Key words: Temperature, Germination, Maize, Gram, Ascorbate, Biochemical |